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Figure 2 ELISA validation of three serum biomarkers using an independent cohort and their correlation with clinical indices. (A–C) A cohort of 80 serum samples: 30 healthy controls (HC, green) and 50 patients with lupus nephritis (LN, red) were tested by ELISA for the levels of ICAM2, <t>TIMP1</t> and THBS1. (D) The discriminatory abilities of the three serum markers were assessed in distinguishing patients with LN from HCs using receiver operating characteristic (ROC) curve analysis. (E–K) The correlation of ELISA-assayed serum ICAM2 levels with clinical indices in LN. r denotes Spearman’s correlation coefficient. ****p<0.0001. AUC, area under the curve.
Serum Samples For Timp1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, <t>TIMP1,</t> ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.
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Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, <t>TIMP1,</t> ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.
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Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, <t>TIMP1,</t> ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.
Anti Timp1 Ba3727, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, <t>TIMP1,</t> ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.
Rabbit Anti Timp1 Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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timp1 monoclonal antibody  (Boster Bio)
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Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, <t>TIMP1,</t> ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.
Timp1 Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2 ELISA validation of three serum biomarkers using an independent cohort and their correlation with clinical indices. (A–C) A cohort of 80 serum samples: 30 healthy controls (HC, green) and 50 patients with lupus nephritis (LN, red) were tested by ELISA for the levels of ICAM2, TIMP1 and THBS1. (D) The discriminatory abilities of the three serum markers were assessed in distinguishing patients with LN from HCs using receiver operating characteristic (ROC) curve analysis. (E–K) The correlation of ELISA-assayed serum ICAM2 levels with clinical indices in LN. r denotes Spearman’s correlation coefficient. ****p<0.0001. AUC, area under the curve.

Journal: Lupus science & medicine

Article Title: Proteomics uncovers ICAM2 (CD102) as a novel serum biomarker of proliferative lupus nephritis.

doi: 10.1136/lupus-2024-001446

Figure Lengend Snippet: Figure 2 ELISA validation of three serum biomarkers using an independent cohort and their correlation with clinical indices. (A–C) A cohort of 80 serum samples: 30 healthy controls (HC, green) and 50 patients with lupus nephritis (LN, red) were tested by ELISA for the levels of ICAM2, TIMP1 and THBS1. (D) The discriminatory abilities of the three serum markers were assessed in distinguishing patients with LN from HCs using receiver operating characteristic (ROC) curve analysis. (E–K) The correlation of ELISA-assayed serum ICAM2 levels with clinical indices in LN. r denotes Spearman’s correlation coefficient. ****p<0.0001. AUC, area under the curve.

Article Snippet: Serum samples for TIMP1 (CUSABIO, Catalogue No CSB- E08003h, Wuhan, China) and THBS1 (CUSABIO, Catalogue No CSB- E08763h, Wuhan, China) were diluted 1:100 and 1:400, respectively, whereas the ICAM2 (BOSTER, Catalogue No EK0999, Peachtree Corners, Georgia) ELISA assays used serum at 1:5 dilution.

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, TIMP1, ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.

Journal: International Journal of Medical Sciences

Article Title: Establishment of a nomogram model based on immune-related genes using machine learning for aortic dissection diagnosis and immunomodulation assessment

doi: 10.7150/ijms.100572

Figure Lengend Snippet: Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, TIMP1, ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.

Article Snippet: CXCL1 (Elab Sciences, #E-EL-H0045), TIMP1 (Elab Science, #E-EL-H0184), ITGA5 (Boster Bio, #EK2227), and PTX3 (Invitrogen, #EH386RB) ELISA Kit were used to measure the concentration of each protein marker in serum.

Techniques: Biomarker Discovery, Expressing, Control, Concentration Assay